The mechanism of oxidative hemolysis produced by phenylhydrazine
Goldberg, B.; Stern, A.
Molecular Pharmacology 13(5): 832-839
1977
ISSN/ISBN: 0026-895X PMID: 895719 Document Number: 113786
rapidly and converted HbO2 into methemoglobin, hemichromes and other Hb breakdown products. Phenyldiazene-induced hemolysis requires the presence of molecular O2, although superoxide anion, a known product of the reaction of phenyldiazene with O2, was demonstrated not to be the active hemolytic agent. The hemolysis produced in the presence of phenyldiazene and O2 is related to lipid peroxidation in the red cell membrane. Benzenediazonium ion and benzene, oxidation products of phenylhydrazine, were not hemolytic when compared with equimolar quantities of phenyldiazene. The active hemolytic agent produced from phenylhydrazine in red cells is a phenyldiazene-derived free radical produced by the reaction of phenyldiazene with O2, and is most probably the phenyl free radical.