Stimulation of gastrin release and gastric secretion by amino acids bathing pyloric gland area
Konturek, S.J.; Tasler, J.; Cieszkowski, M.; Dobrzańska, M.; Wünsch, E.
American Journal of Physiology 233(3): E170-E174
1977
ISSN/ISBN: 0002-9513 PMID: 910904 Document Number: 113278
The potency was tested of L- and D-isomers of 18 natural amino acids bathing the pyloric gland area for stimulation of gastrin release and gastric acid secretion in 3 dogs with denervated antral (AP) and Heidenhain pouches (HP) and gastric fistulae (GF). L-Isomers of all natural amino acids stimulated acid secretion from the HP, whereas D-isomers were less effective. The most potent L-isomers of amino acids were phenylalanine, alanine and glycine, which increased acid outputs, reaching 36, 29, and 26% of the maximum response to histamine (MRH). The strongest stimulants of acid secretion were relatively weak releasers of gastrin. Irrigation of the AP with increasing concentrations of L-phenylalanine 0.1 to 0.8 mol/litre, liver extract 2.5 to 20% and acetylcholine 0.1 to 0.8% increased acid outputs stepwise, reaching 31, 22, and 12% of the MRH. Three gastrin peptides, G-14, G-17 and G-34, infused in graded doses from 6.2 to 100 pmol/kg h gave a dose-related increase in acid output, reaching 25, 17, and 17% of the MRH. The increment in serum gastrin was also dose-related and similar with G-14 and G-17 but significantly more with G-34.