Molecular changes in lymph node tissue after skin heterografts
Rifé, S.U.
Acta Physiologica Latino Americana 27(3): 114-120
1977
ISSN/ISBN: 0001-6764 PMID: 356521 Document Number: 111860
Changes in the rat lymph node RNA base composition and in ribosomal aggregation induced by heterologous skin graft have been characterized by the base composition analysis of the phenol extractable RNA, by the density gradient centrifugation of the ribosomal particles and by amino acid incorporation in the newly synthetized protein. Following skin grafts, the changes in base composition of total RNA in lymph . nodes were mainly due to the synthesis of ribosomal type RNA. Ribosomes were assembled as single units or as polyribosomes. The present findings also show an increase in amino acid incorporation by polyribosomes after the first graft reaction.