Membrane characteristics of adherent cells dissociated from rheumatoid synovial tissue
Abrahamsen, T.G.; Johnson, P.M.; Natvig, J.B.
Clinical and Experimental Immunology 28(3): 474-483
1977
ISSN/ISBN: 0009-9104 PMID: 330058 Document Number: 110934
Synovial tissues from rheumatoid-arthritis patients were dissociated by enzymes and the resulting cells incubated overnight in tissue-culture flasks. The adherent cell population was resuspended with EDTA-trypsin, and morphological examination showed 68-80% non-lymphoid cells, most of which had the appearance of synovial lining cells. The proportion of these cells increased during subsequent culture. Between 40 and 60% of the cells exhibited marked phagocytosis, and < 14% of the non-lymphoid cells could form EA G or .alpha.2-macroglobulin also gave similar results, whereas the F(ab')2 fragment of rabbit IgG antibody to human .alpha.2-macroglobulin was completely negative. More than 99% of the cells commonly exhibited membrane-associated .beta.2-microglobulin.