The action of cysteine on PHA-stimulation of lymphocytes. I. Influence of DNA synthesis
Ryzewski, J.; Roszkowski-Sliz, W.; Krzystyniak, K.
Archivum Immunologiae et Therapiae Experimentalis 25(3): 377-383
1977
ISSN/ISBN: 0004-069X PMID: 889426 Document Number: 110376
The influence of short-time preincubation of rat lymphocytes in solutions of L-cysteine reduced glutathione, and dithiothreitol on PHA stimulation of these cells was studied by measuring incorporation of 3H-thymidine. Thirty-minute preincubation in cysteine solution enhanced PHA-induced incorporation of 3H-thymidine. One-hour incubation caused earlier peak of incorporation by one day; and two-hour incubation distinctly inhibited spontaneous incorporation of 3H-thymidine. Incubation in solutions of glutathione and dithiothreitol had no effect on the course or intensity of 3H-thymidine incorporation. However, constant presence of these compounds in cultures was toxic for lymphocytes.