Histologic and immunologic study of the testis after ligation of the vas deferens in dogs
Lansac, J.; Chavrier, F.; Grimaud, J.A.; André, D.
Journal de Gynecologie Obstetrique et Biologie de la Reproduction 5(1): 5-14
1976
ISSN/ISBN: 0368-2315 PMID: 956613 Document Number: 110023
We wanted to study the effect of bilateral ligature and section of the deferentia of 12 dogs from the histological and immunological aspects. We looked for circulating anti-sperm antibodies and anti-basal membrane of the testes antibodies at the same time as histological study to find out what happened to these lesions after epididymo-deferential anastomosis had been carried out 16 weeks later. The histological lesions (disappearance of exfoliation of the germ cells and of necrobiosis of these cells) disapperared after anastomosis. Anti-basal membrane antibodies were never found. Anti-testicular circulating auto-antibodies were never found. Anti-sperm antibodies appeared between 1 and 5 months after ligature and then disappeared a few months later. Failures to fertilise after epiddidymo-deferential anastomoses therefore do not seem to be due to these two factors.