Chloride transport in isolated frog (Rana temporaria) skin: changes in short-circuit currently, potential, resistance, and chloride flux elicited by furosemide
Lote, C.J.
Pflugers Archiv European Journal of Physiology 362(2): 181-184
1976
ISSN/ISBN: 0031-6768 PMID: 1083503 Document Number: 109470
In a large number of isolated frog skins, with potential differences of from 20-92 mV (mean 55.3 .+-. 3.6 SE of the mean) the Cl- influx was slightly greater than Cl- efflux under short-circuit conditions, but the difference was not statistically significant. However, if skins of low potential (less than 50 mV) were selected, Cl- influx was significantly higher than Cl- efflux (P < 0.05). In this low potential group furosemide, (10-3 M, applied to the solution bathing the mucosal surface) produced a small increase in short-circuit current, which was generally apparent within 1 min, and maximal in 5 min; and thereafter declined towards the control value; a marked increase in potential difference, apparent within 1 min and sustained for at least 30 min; a large and sustained increase in the calculated DC resistance of the skin and a decrease in the influx of Cl-, such that influx and efflux were equalized.