Nuclear protein kinase of brain: effect of S-100 protein

Perumal, A.S.

Research Communications in Chemical Pathology and Pharmacology 13(3): 489-496

1976


ISSN/ISBN: 0034-5164
PMID: 180574
Document Number: 102205
The non-histone chromosomal protein fraction isolated from purified brain nuclei possesses protein kinase activity. 93% of this activity is lost by heating at 80 degrees C for 5 min. cAMP does not affect the reaction, but cGMP is inhibitory. In the presence of S-100, an acidic brain-specific protein, phosphate incorporation is enhanced 3 to 4 fold. Bovine serum albumin has no effect whereas histone inhibits activity.

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