Effects of streptozotocin added in vitro on insulin biosynthesis and release in isolated rat islets
Maldonato, A.; Trueheart, P.; Renold, A.E.
Hormone and Metabolic Research 6: 71-73
1976
ISSN/ISBN: 0018-5043 PMID: 132395 Document Number: 100702
The effect of different concentrations of streptozotocin was tested in vitro on isolated rat islets. The following parameters of islet-cell activity were measured: 1) incorporation of 3H-leucine into proinsulin and into total islet protein; 2) IRI release into the medium, and 3) islet proinsulin content. It appears from the preliminary results shown here that both insulin release and biosynthesis were decreased by streptozotocin in a way which was dose- and time-dependent.
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